Partnering with CytoSolve

Nutritional Supplement, Ingredients and Functional Food Manufacturers

The CytoSolve platform enables product manufacturers such as nutritional supplement companies, ingredient suppliers, functional food companies etc. to gain insights to understand how particular ingredients and/or single molecules act upon a particular indication or molecular mechanism of action. The detailed insights provide manufacturers and ingredient suppliers a powerful capability to determine how to formulate truly efficacious products and articulate the value of those ingredients and formulations to their customers and consumers.

CytoSolve’s technology affords industry partners a unique opportunity to

ACHIEVE 5 SPECIFIC GOALS

1. EXCELLENCE  IN  PRODUCT DEVELOPMENT

by accessing and commissioning research on the molecular mechanistic understanding of particular ingredient and/or single molecule on a particular indication e.g. curcumin on inflammation, through In Silico Ingredient (ISI) testing.

2. VALIDATION  OF  PRODUCT  COMBINATIONS

to understand not only their combined efficacy on a given indication, but also what ingredients are, and what ingredients are not, contributing to that efficacy through In Silico Combination (ISC) testing.

3. OPTIMIZATION OF PRODUCT COMBINATIONS

by identifying, for an existing product, what new dosages of ingredients may provide equal efficacy at a lower cost “bang for the buck” through In Silico Optimization (ISO).

4. DISCOVERY OF  NEW  PRODUCTS

by formulating a completely new combinations that are optimal for given indications while modulating price and efficacy through In Silico Discovery (ISD).

5. INNOVATION

through participation in a CytoSolve Research Consortium that ensures scientific leadership in a dynamic environment where scientific knowledge and advances are accelerating at a pace where an individual scientist and/or a scientific research team are incapable of assimilating the voluminous information in any field.

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In Silico Efficacy Analysis of Dietary Supplements

Dietary supplements contain different active ingredients, and enzymes. Effect of each ingredient and enzymes on different molecular cascades are identified through systematic literature review. Individual effect of ingredients and enzymes on different signaling pathways are transformed into computational models. Using CytoSolve platform, the additive or synergistic effect of the active ingredients are calculated for different dose of food supplements.

CYTOSOLVE CASE STUDY: Systems Health

Systems HealthComputational modeling of signaling pathways is an approach at CytoSolve that provides insight into various physiological processes. Memory and cognition are fascinating processes in human behavior. Understanding the molecular mechanisms involved in these processes paves the way for development of strategies for memory enhancement. The CytoSolve approach in this direction involves the transformation of signaling pathways in learning and memory into computational models. The impact of nutraceuticals on the backbone models could be tested with the help of CytoSolve scalability approach. The ultimate aim is to formulate optimized nutraceutical combinations for the enhancement of memory and cognition.

CYTOSOLVE CASE STUDY: Solid Gold® Pet Food

Solid Gold® Pet FoodThere is a body of empirical evidence demonstrating the positive effect of dietary supplement Solid Gold® SeaMeal™ on coat appearance of pets. However, the molecular mechanistic understanding of the effect of SeaMeal ingredients, individually and in combination is not clearly understood. In this study, a systematic literature review is conducted to identify the molecular pathways affecting pet coat appearance. The molecular pathways of coat appearance are converted to individual mathematical models; each model is validated; and, the plurality of models is integrated with the CytoSolve® computational systems biology platform to produce an integrative model of coat appearance. CytoSolve provides for the dynamic integration of molecular pathway models, in silico, to understand synergistic effects of multi-ingredient dietary supplements on molecular pathways of biological processes. Five individual ingredients from Solid Gold® SeaMeal™, specifically, bladderwrack kelp, dulse flakes kelp, flaxseed, alpha amylase and bromelain, are tested, in silico using the CytoSolve model, to evaluate efficacy. Combination of the five ingredients, from the current dosage in SeaMeal, is also tested in silico. The results from the systematic review reveal two major molecular pathway systems that govern coat appearance: 1. cornified envelope synthesis and 2. oxidative stress pathways in skin. The results from the CytoSolve in silico modeling demonstrate that SeaMeal ingredients synergistically enhance coat appearance by: 1.increasing cornified envelope synthesis, and 2. reducing the oxidative stress in the skin.

Toxicity Analysis of Supplements

Diverse responses of target or biomarker induced toxicity through different signaling pathways are identified through systematic literature review. Computational models are constructed to identify the impact of each drug or ingredients on susceptible target or biomarker. CytoSolve platform evaluates the efficacy as well as toxicity of drugs or ingredients in combination. Dose of either drug and/or ingredients in combinations are tested to identify the threshold dose.

CYTOSOLVE CASE STUDY: Safety Testing for United States Pharmacopeia

Safety Testing for United States PharmacopeiaUse of dietary supplements by military personnel is well acknowledged and documented in a review conducted by the Institute of Medicine. About half of the US population uses dietary supplements. Concerns regarding the benefits and safety of dietary supplement use by military arise because (a) military personnel may have a different intent than the general population and (b) military personnel have unique challenges and needs. CytoSolve® computational systems biology platform provides for the dynamic integration of molecular pathway models, in silico, to understand synergistic effects of multi-ingredient dietary supplements on molecular pathways of biological processes. CytoSolve platform was used to evaluate the effect of combinations of arginine and caffeine, found in dietary supplements taken by military personnel, on nitric oxide production, which is directly related to cardiovascular health. The CytoSolve analysis revealed that arginine alone increased the nitric oxide concentration whereas caffeine alone reduced the nitric oxide concentration in healthy individuals. In combination, the results indicated that production rate in healthy individuals did not increase significantly by arginine supplementation. On the other hand, arginine supplementation attenuated NO production rate to nearly normal levels in hyperglycemic/hypertensive individuals.

National Animal Supplement Council

Efficacy Analysis of Functional Foods

The nutritive value of foods is defined by the type and proportion of bioactive compounds present. Commonly found classes of bioactive compounds in foods include polyphenols, flavanoids, saponins, carotenoids, anthocyanins, vitamins, essential oils and enzymes. Optimum conditions are necessary for these compounds to exhibit their numerous health benefits. Analysis of the composition, thermal stability and pH stability of active compounds in foods enables the study of the effects of processing steps on their functional efficacy.

CYTOSOLVE CASE STUDY: Two Moms

Two MomsGenerally foods undergo different processing steps to stop or deteriorate chemical or biochemical reactions; eliminate infestation of food with microbes, insects or other vermins; and to increase storage stability. Nuts or seeds are generally roasted to be freed from microorganisms and for long term preservation. TMITR has consulted CytoSolve to identify the health benefits of foods with different processing steps like germination and roasting. Systematic literature review are executed to identify the composition of active ingredients from almonds, sunflower seeds, and oat groats. Detailed composition of nutraceutical of different varieties and species from different geographical locations are documented. CytoSolve research methodology is used to uncover the comparative differences between raw, germinated and roasted food. The systematic analysis of active ingredients from nutraceutical also evaluate the risk factors and health benefits for the consumption of processed foods.

CYTOSOLVE CASE STUDY: Hail Merry

Hail MerryMore and more people are incorporating what they believe to be raw foods into their diets principally for health and taste reasons. But there is debate and confusion over what actually constitutes “raw” and what the characteristics should be to maintain a food’s ‘raw’ quality at point of sale and also protect consumers from foodborne diseases. International Center for Integrative Systems has developed a standard that aims to verify the claims and support the growth of the raw food community while enabling transparency for consumers, retailers, manufacturers and the regulatory community. Towards the certification of raw products manufactured by Hail Merry, the list of names and weight percentage of all ingredients in each product, and the processing performed for each product were acquired from Hail Merry Foods. A systematic literature review was conducted to identify active ingredient molecules from different categories like polyphenols, flavanoids, saponins, carotenoids, anthocyanins, vitamins, essential oils and enzymes. Active ingredients identified are analyzed with their physical, chemical and physiological properties. Using the proprietary Bioavailability model developed by CytoSolve, bioavailability was estimated for each ingredient. The bioavailability score for each product was calculated as a function of bioavailability of all the ingredients and the weight percentage of each ingredient. A score of >80% is required to fulfil the Bioavailability criteria of the Raw Food Standard. The bioavailability analysis was performed on 20 products from Hail Merry Foods.

CYTOSOLVE CASE STUDY: Brad’s Raw Foods

Brad’s Raw FoodsMore and more people are incorporating what they believe to be raw foods into their diets principally for health and taste reasons. But there is debate and confusion over what actually constitutes ‘raw’ and what the characteristics should be to maintain a food’s ‘raw’ quality at point of sale and also protect consumers from foodborne diseases. International Center for Integrative Systems has developed a standard that aims to verify the claims and support the growth of the raw food community while enabling transparency for consumers, retailers, manufacturers and the regulatory community. Towards the certification of raw products manufactured by Brad’s Raw Foods, the list of names and weight percentage of all ingredients in each product, and the processing performed for each product were acquired from Brad’s Raw Foods. A systematic literature review was conducted to identify top 3-5 active ingredient molecules representing the significant micronutrients were identified along with their physical, chemical and physiological properties. Using the proprietary Bioavailability model developed by CytoSolve, bioavailability was estimated for each ingredient. The bioavailability score for each product was calculated as a function of bioavailability of all the ingredients and the weight percentage of each ingredient. A score of >80% is required to fulfil the Bioavailability criteria of the Raw Food Standard. The bioavailabilty analysis was performed on 13 products from Brad’s Raw Foods. All of the products score more that 80% (86-100%) and passed the Bioavailability criteria.